The Challenges of Vaccine Growth towards Betacoronaviruses: Antibody Dependent Enhancement and Sendai Virus as a Doable Vaccine Vector
To design an efficient and protected vaccine towards betacoronaviruses, it’s vital to make use of their evolutionarily conservative antigenic determinants that can elicit the mix of robust humoral and cell-mediated immune responses. Concentrating on such determinants minimizes the chance of antibody-dependent enhancement of viral an infection.
This phenomenon was noticed in animal trials of experimental vaccines towards SARS-CoV-1 and MERS-CoV that had been developed primarily based on inactivated coronavirus or vector constructs expressing the spike protein (S) of the virion.
The substitution and glycosylation of sure amino acids within the antigenic determinants of the S-protein, in addition to its conformational adjustments, can result in the identical impact in a brand new experimental vaccine towards SARS-CoV-2. Utilizing extra conservative structural and accent viral proteins for the vaccine antigenic determinants will assist to keep away from this downside.
This assessment outlines approaches for growing vaccines towards the brand new SARS-CoV-2 coronavirus which are primarily based on non-pathogenic viral vectors. For environment friendly prevention of infections brought on by respiratory pathogens the flexibility of the vaccine to stimulate mucosal immunity within the respiratory tract is essential. Such a vaccine may be developed utilizing non-pathogenic Sendai virus vector, since it may be administered intranasally and induce a mucosal immune response that strengthens the antiviral barrier within the respiratory tract and gives dependable safety towards an infection.

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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
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PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 770.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
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11010 | lumiprobe | 100 uL | EUR 59.00 |
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61010 | lumiprobe | 2 mL | EUR 363.00 |
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51010 | lumiprobe | 1.5 mL | EUR 331.00 |
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41010 | lumiprobe | 1 mL | EUR 249.00 |
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81010 | lumiprobe | 10 mL | EUR 869.00 |
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71010 | lumiprobe | 5 mL | EUR 625.00 |
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91010 | lumiprobe | 50 mL | EUR 3169.00 |
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Growth of Excessive Decision DNA Melting Evaluation for Simultaneous Detection of Potato Mop-High Virus and Its Vector, Spongospora subterranea, in Soil
On this examine, a set of duplex reverse transcription (RT)-PCR-mediated excessive decision DNA melting (HRM) analyses for simultaneous detection of potato mop-virus (PMTV) and its protist vector, Spongospora subterranea f.sp. subterranea (Sss), was developed.
- The infestation of soil by PMTV was detected by utilizing a tobacco-based baiting system. Whole RNA extracted from the soil led to profitable RT-PCR gel-electrophoresis detection of each PMTV and Sss.
- To facilitate extra environment friendly detection, newly designed primer pairs for PMTV RNA species (i.e., RNA-Rep, -CP, and -TGB) had been analyzed along with the present Sss primers utilizing real-time RT-PCR. The ensuing amplicons exhibited melting profiles that may very well be readily differentiated.
- Underneath duplex RT-PCR format, all PMTV and Sss primer mixtures led to profitable detection of respective PMTV RNA species and Sss within the samples by excessive decision DNA melting (HRM) analyses.
- When the duplex HRM assay was utilized to soil samples collected from six fields at 4 totally different websites in New Brunswick, Canada, optimistic detection of PMTV and/or Sss was present in 63-100% samples collected from fields during which PMTV-infected tubers had been noticed.
- In distinction, the samples from fields the place neither PMTV- nor Sss-infected tubers had been noticed resulted in detrimental detection by the assay. Bait tobacco bioassay for PMTV and Sss produced related outcomes.
- Between 63%-83% and 100% of the soil samples collected from PMTV-infested fields led to PMTV and Sss infections within the bait tobacco vegetation, respectively; whereas no PMTV or Sss contaminated vegetation had been obtained from soil samples collected from PMTV/Sss-free fields.
The Brighton Collaboration standardized template for assortment of key data for benefit-risk evaluation of viral vector vaccines
Lots of the vaccines underneath improvement for COVID-19 contain using viral vectors. The Brighton Collaboration Profit-Threat Evaluation of Vaccines by Expertise (BRAVATO, previously the Viral Vector Vaccine Security Working Group, V3SWG) working group has ready a standardized template to explain the important thing concerns for the benefit-risk evaluation of viral vector vaccines. It will facilitate key stakeholders to anticipate potential security points and interpret or assess security knowledge. This might additionally assist enhance communication and public acceptance of licensed viral vector vaccines.
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11010 | lumiprobe | 100 uL | EUR 59.00 |
![]() dsGreen for Real-Time PCR, 100×, 2 mL |
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61010 | lumiprobe | 2 mL | EUR 363.00 |
![]() dsGreen for Real-Time PCR, 100×, 1.5 mL |
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51010 | lumiprobe | 1.5 mL | EUR 331.00 |
![]() dsGreen for Real-Time PCR, 100×, 1 mL |
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41010 | lumiprobe | 1 mL | EUR 249.00 |
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81010 | lumiprobe | 10 mL | EUR 869.00 |
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71010 | lumiprobe | 5 mL | EUR 625.00 |
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91010 | lumiprobe | 50 mL | EUR 3169.00 |
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MB1004-100Reactions | BosterBio | 100 Reactions | EUR 327.00 |
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31033 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31034 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31035 | Biotium | 1kit | EUR 399.00 |
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31036 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31050 | Biotium | 1kit | EUR 428.00 |
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31051 | Biotium | 1kit | EUR 399.00 |
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31053 | Biotium | 1kit | EUR 428.00 |
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31033-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31050-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31051-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
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RR-0478-02 | Liferiver | 25 tests/kit | EUR 991.00 |
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
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31037 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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DD2H | Detroit R&D | 1 Kit | EUR 430.00 |
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DD2M | Detroit R&D | 1 Kit | EUR 430.00 |
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DD2R | Detroit R&D | 1 Kit | EUR 430.00 |
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20-abx097601 | Abbexa |
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M034-Kit | TOKU-E | Kit | EUR 266.00 |
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HLUCPL-I | Real Time Primers | 1 set | EUR 548.00 |
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HSCH-I | Real Time Primers | 1 set | EUR 548.00 |
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A26979 | GeneOn | 1x100 pcs | EUR 186.00 |
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FYT105-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx |
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31037-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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FYT103-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
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FYT106-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
|||
PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 709.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
|||
PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 770.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097603 | Abbexa |
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![]() hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097604 | Abbexa |
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![]() hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097605 | Abbexa |
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![]() hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097616 | Abbexa |
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![]() hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097617 | Abbexa |
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![]() hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097618 | Abbexa |
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![]() hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097619 | Abbexa |
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![]() hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097620 | Abbexa |
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![]() hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097622 | Abbexa |
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![]() hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097625 | Abbexa |
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![]() hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097626 | Abbexa |
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![]() hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097627 | Abbexa |
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![]() hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097628 | Abbexa |
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![]() hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097629 | Abbexa |
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![]() hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097630 | Abbexa |
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![]() hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097631 | Abbexa |
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![]() hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097632 | Abbexa |
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![]() hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097633 | Abbexa |
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![]() hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097634 | Abbexa |
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![]() hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097636 | Abbexa |
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![]() hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097637 | Abbexa |
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![]() hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097638 | Abbexa |
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![]() hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097639 | Abbexa |
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![]() hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097640 | Abbexa |
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![]() hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097642 | Abbexa |
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![]() hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097643 | Abbexa |
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![]() hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097644 | Abbexa |
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![]() hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097645 | Abbexa |
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![]() hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097647 | Abbexa |
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![]() hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097648 | Abbexa |
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![]() hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097650 | Abbexa |
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![]() hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097651 | Abbexa |
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![]() hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097652 | Abbexa |
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![]() hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097653 | Abbexa |
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![]() hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097654 | Abbexa |
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![]() hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097657 | Abbexa |
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![]() hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097659 | Abbexa |
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![]() hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097661 | Abbexa |
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![]() hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097662 | Abbexa |
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![]() hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097663 | Abbexa |
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![]() hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097664 | Abbexa |
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![]() hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097665 | Abbexa |
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![]() hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097666 | Abbexa |
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![]() hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097667 | Abbexa |
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![]() hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097669 | Abbexa |
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![]() hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097671 | Abbexa |
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![]() hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097673 | Abbexa |
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![]() hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097675 | Abbexa |
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![]() hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097676 | Abbexa |
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![]() hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097677 | Abbexa |
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![]() hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097678 | Abbexa |
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![]() hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097679 | Abbexa |
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![]() hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097680 | Abbexa |
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![]() hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097681 | Abbexa |
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![]() hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097686 | Abbexa |
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![]() hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097689 | Abbexa |
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![]() hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097690 | Abbexa |
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![]() hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097691 | Abbexa |
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![]() hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097692 | Abbexa |
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![]() hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097693 | Abbexa |
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![]() hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097694 | Abbexa |
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![]() hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097695 | Abbexa |
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![]() hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097696 | Abbexa |
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![]() hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097699 | Abbexa |
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![]() hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097700 | Abbexa |
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![]() hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097701 | Abbexa |
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![]() hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097702 | Abbexa |
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![]() hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097703 | Abbexa |
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![]() hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
20-abx097706 | Abbexa |
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![]() hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097708 | Abbexa |
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![]() hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
20-abx097709 | Abbexa |
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![]() hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
20-abx097711 | Abbexa |
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